Liposome mediated gene transfer into the rat oesophagus
نویسندگان
چکیده
منابع مشابه
Liposome mediated gene transfer into the rat oesophagus.
BACKGROUND Cancer of the oesophagus has so far eluded every attempt at pharmacological treatment. The recent advent of somatic gene therapy offers a new therapeutic approach to malignant tumours. AIM To investigate whether and how gene transfer into the oesophagus can be achieved. METHODS A LacZ reporter gene was used as marker and transferred into the oesophagus of rats using cationic lipo...
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BACKGROUND Complexing recombinant DNA with cationic liposomes is a convenient means of introducing foreign genes into cells (lipofection) and could potentially form the basis for genetically modifying diseased blood vessels in patients. The mechanism of lipofection is incompletely understood, but it is recognized that the degree of successful gene transfer is highly dependent on cell type. To d...
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Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...
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ژورنال
عنوان ژورنال: Gut
سال: 1997
ISSN: 0017-5749,1468-3288
DOI: 10.1136/gut.41.4.549